ctrp3 levels (Aviva Systems)
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Aviva Systems
ctrp3 levels
Ctrp3 Levels, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctrp3+levels/pmc06076208-87-1-9?v=Aviva+Systems
Average 88 stars, based on 1 article reviews
Ctrp3 Levels, supplied by Aviva Systems, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ctrp3+levels/pmc06076208-87-1-9?v=Aviva+Systems
Average 88 stars, based on 1 article reviews
ctrp3 levels - by Bioz Stars,
2026-07
88/100 stars
Images
1) Product Images from "Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction"
Article Title: Melatonin improves cardiac function in a mouse model of heart failure with preserved ejection fraction
Journal: Redox Biology
doi: 10.1016/j.redox.2018.07.007
Figure Legend Snippet: Melatonin increased myocardial CTRP3 expression in obese mice. (A-B) Representative blots and results for myocardial CTRP3 expression (n = 6 per group). (C) Representative images of myocardial CTRP3 immunostaining. (D-E) CTRP3 expression in H9c2 cells. H9c2 cells were treated with melatonin for 15 min, after which they were collected for blot detection. Data are expressed as the mean ± SD of six independent experiments (D-E). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05 compared with the ND+vehicle group, # P < 0.05 compared with the HFD+vehicle group.
Techniques Used: Expressing, Immunostaining
Figure Legend Snippet: Melatonin caused adipocytes to secrete CTRP3 without affecting adiponectin expression or inflammation. (A) CTRP3 expression in 3T3-L1 adipocytes. 3T3-L1 adipocytes were subjected to treatment with melatonin (10 μmol/L) at different time points and then collected for blot detection. (B-C) Representative images and results for CTRP3 immunostaining in adipose tissue after melatonin treatment (n = 5 per group). (D) Circulating CTRP3 levels after melatonin treatment (n = 6 per group). (E) The level of adiponectin in adipose tissues (n = 5 per group). Data are expressed as the mean ± SD of six independent experiments (A). Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
Techniques Used: Expressing, Immunostaining
Figure Legend Snippet: Depleting circulating CTRP3 using a CTRP3 antibody largely abolished melatonin-mediated protection against diastolic dysfunction. (A) LVEDP after melatonin treatment (n = 5 per group). (B) -dP/dt with melatonin treatment (n = 5 per group). (C) Alterations in the Tau index after melatonin treatment (n = 5 per group). (D) The level of 4-HNE (n = 5 per group). (E-F) The mRNA levels of Sod2 and Gpx (n = 5 per group). All data are expressed as the mean ± SD. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
Techniques Used:
Figure Legend Snippet: Adipocyte-derived CTRP3 activated Nrf2 to prevent oxidative stress and cell apoptosis. (A) CTRP3 expression in the pooled adipocyte medium. 3T3-L1 adipocytes were treated melatonin (10 μmol/L) or vehicle for 24 h, after which the medium was collected to detect CTRP3 expression. ConM-V: conditioned medium in which adipocytes treated with vehicle were cultured; ConM-M: conditioned medium in which adipocytes treated with melatonin were cultured. (B-C) Nrf2, HO-1 and SOD2 expression in H9c2 cells. H9c2 cells were exposed to ConM-M for different time periods and then collected for blot detection. (D) Immunostaining of Nrf2 in H9c2 cells. H9c2 cells were exposed to ConM-V or ConM-M for 4 h and then collected for blot detection. (E-F) CTRP3 deficiency in adipocytes largely abolished the effects ConM-M on Nrf2 and HO-1 expression in H9c2 cells. (G) Cell viability in all the groups. (H) Sod2 mRNA levels in H9c2 cells. (I) DCFH-DA staining and TUNEL staining. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
Techniques Used: Derivative Assay, Expressing, Cell Culture, Immunostaining, Staining, TUNEL Assay, Recombinant
Figure Legend Snippet: miR-200a was closely associated with Nrf2 activation. (A) Nrf2-related miRNA levels. H9c2 cells were exposed to ConM-V or ConM-M for 24 h and then collected for further analysis. (B) miR-93a, miR-193b and miR-200a levels after rhCTRP3 treatment. (C) Keap1 mRNA levels. (D–F) Nrf2 and HO-1 protein levels. rhCTRP3, recombinant human CTRP3. All data are expressed as the mean ± SD of six independent experiments. Differences were compared by one-way ANOVA followed by Tukey's post hoc test. * P < 0.05.
Techniques Used: Activation Assay, Recombinant
